A transcriptionally regulated expression vector for the fission yeast Schizosaccharomyces pombe.
نویسندگان
چکیده
An expression vector for the fission yeast Schizosaccharomyces pombe is described. The vector is designed to facilitate the construction of transcriptional fusions to the promoter of the S. pombe fructose bisphosphatase gene. Transcription from this promoter is regulated by glucose repression over a range of greater than 100-fold. The tight regulation by this promoter should allow for the maintenance of genes whose products are lethal to S. pombe and for the high level production of their protein or RNA products. Intermediate levels of expression can also be achieved by growth on different carbon sources.
منابع مشابه
Construction of an expression vector for the fission yeast Schizosaccharomyces pombe.
We have isolated and characterized a S. pombe promoter using a functional heterologous gene product assay. Random S. pombe genomic fragments were cloned upstream from the promoterless 'lacZ gene and tested in vivo for their efficiency to promote expression of the beta-galactosidase protein in the fission yeast. An efficient S. pombe promoter called 54/1 was isolated and shown to drive up to 5% ...
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عنوان ژورنال:
- Gene
دوره 84 2 شماره
صفحات -
تاریخ انتشار 1989